anti nectin2 mab  (R&D Systems)

Journal: Journal for Immunotherapy of Cancer

Article Title: Interactions between LAMP3+ dendritic cells and T-cell subpopulations promote immune evasion in papillary thyroid carcinoma

doi: 10.1136/jitc-2024-008983

Figure Lengend Snippet: The recruitment of LAMP3 + DCs by tumor cells promotes PTC clinical progression. Heatmaps (A) and circle (B) plots show the comparison of interaction quantity and interaction strength between thyrocytes and cDCs between non-progressive PTC and progressive PTC. Red indicated that the increase of communication in the latter. (C) The interaction between thyrocytes and cDCs is more numerous and stronger in Group B. (D) Summary of selected ligand-receptor interactions between thyrocytes and cDC-C3 cells in the two groups. (E) Circle plots showing the interaction between NECTIN3-NECTIN2 ligand-receptor pairs in the cDCs and thyrocytes. (F) The expression level of S100A2 is positively correlated with LAMP3. (G) Representative immunofluorescence images illustrating the interaction between thyrocytes and cDC_C3 in two groups (A1 and B9). The small panels show the magnification of the selected region highlighted in red. Scale bars correspond to 50 µm in the large panel. (H) Schematic showing the crosstalk among thyrocytes, LAMP3 + DCs, CD8 + T cells, and Tregs involved in the recruitment of immune cells and the formation of an immunosuppressive microenvironment in the progressive PTC. cDC. conventional DC; DC, dendritic cell; CTLA-4, cytotoxic T-lymphocyte associated protein 4; ICAM, intercellular adhesion molecule 1; LAMP3, lysosomal associated membrane protein 3; PTC, papillary thyroid cancer; SPN, sialophorin; TME, tumor microenvironment; Treg, regulatory T cell.

Article Snippet: The following antibodies were used in the current study: primary antibodies against DC_LAMP/CD208 (1:400, CST, Cat#47778), CD8A (1:100 dilution; Abcam, Cat#ab217344), TIGIT (1:1,000, Cell Signaling Technology, Cat#99567T), NECTIN2 (1:400, Cell Signaling Technology, Cat#95333T), NECTIN3 (1:500, R&D, Cat#AF3064-SP), CCL17 (1:1,000, Abcam, Cat#ab195044), KRT19 (1:2,000, Abcam, Cat#ab76539), FOXP3 (1:2,000, Abcam, Cat#ab215206), or CCR4 (1:2,000, Novus Biologicals, Cat#NBP1-86584).

Techniques: Comparison, Expressing, Immunofluorescence, Membrane

Journal: Cancer Genomics & Proteomics

Article Title: Analysis of Protein–Protein Interactions Identifies NECTIN2 as a Target of N,N-Bis (5-Ethyl-2-hydroxybenzyl) Methylamine for Inhibition of Lung Cancer Metastasis

doi: 10.21873/cgp.20347

Figure Lengend Snippet: Note: The GO system reported NECTIN2 as poliovirus receptor-related 2 (PVRL2).

Article Snippet: The primary antibodies against NECTIN2 (#95333), β-catenin (#8480), vimentin (#5741), snail family transcriptional repressor 1 (SNAI1) (#3879), snail family transcriptional repressor 2 (SNAI2) (#9585), tight junction protein 1 (TJP1) (#8193), β-actin (#4970), and the secondary antibody anti-rabbit IgG (#7074) were acquired from Cell Signaling Technology (Danvers, MA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells

doi: 10.1038/s41598-017-10403-0

Figure Lengend Snippet: PVR and Nectin2 are mainly found as intracellular pool in MM cells. ( a ) CD38 + CD138 + malignant PCs derived from BM aspirates of MM patients (n = 34) were analysed for PVR and Nectin2 surface and total (surface plus intracellular) expression before and after fixation and permeabilization, respectively. Cells were acquired using FACSCanto flow cytometer (BD Biosciences). Each dot represents a single patient, ****p < 0.001, Wilcoxon matched pairs test. ( b ) PVR (left panel) and Nectin2 (right panel) surface and total (surface plus intracellular) expression was analysed on MM cell lines before and after fixation and permeabilization, respectively. Cells were acquired using FACSCalibur flow cytometer (BD Biosciences). Data represent the means ± SD of PVR and Nectin2 from three independent experiments. *p < 0.05, Student T test. MFI: mean fluorescence intensity.

Article Snippet: Surface ligands expression on patient-derived PCs was evaluated by means of PE-conjugated anti-PVR mAb (Biolegend, SKII.4), and APC-conjugated anti-Nectin2 mAb (R&D Systems, FAB2229A) after gating on the CD38 + CD138 + PC population.

Techniques: Derivative Assay, Expressing, Flow Cytometry, Fluorescence

Journal: Scientific Reports

Article Title: Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells

doi: 10.1038/s41598-017-10403-0

Figure Lengend Snippet: SUMOylation controls PVR but not Nectin2 surface expression in MM cell lines. ( a–c ) Inhibition of the SUMO pathway was achieved by means of overnight treatment with 25μg/mL Ginkgolic Acid (GA). The efficacy of treatment was verified by means of western blot analysis on total cell lysates ( a ). ( b , c ) PVR and Nectin2 surface expression was evaluated on ARK and OPM2 cell lines by immunofluorescence and FACS analysis using FACSCalibur flow cytometer (BD Biosciences). One out of three independent experiments ( b ) and means ± SD of PVR and Nectin2 MFI from three independent experiments ( c ) are shown. **p < 0.01, ***p < 0.001, Two-way ANOVA. ( d–f ) Inhibition of the SUMO pathway was achieved by means of UBC9 gene silencing. Silencing efficiency was verified by means of western blot analysis on total cell lysates ( d ). ( e , f ) PVR and Nectin2 surface expression was evaluated on ARK and OPM2 cell lines by immunofluorescence and FACS analysis using FACSCanto flow cytometer (BD Biosciences). One out of three independent experiments ( e ) and means ± SD of PVR and Nectin2 MFI from three independent experiments ( f ) are shown. **p < 0.01, ***p < 0.001, Two-way ANOVA.

Article Snippet: Surface ligands expression on patient-derived PCs was evaluated by means of PE-conjugated anti-PVR mAb (Biolegend, SKII.4), and APC-conjugated anti-Nectin2 mAb (R&D Systems, FAB2229A) after gating on the CD38 + CD138 + PC population.

Techniques: Expressing, Inhibition, Western Blot, Immunofluorescence, Flow Cytometry

Journal: Scientific Reports

Article Title: Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells

doi: 10.1038/s41598-017-10403-0

Figure Lengend Snippet: Ginkgolic Acid treatment up-regulates PVR but not Nectin2 surface expression in malignant PCs. Malignant PCs were treated overnight with 25 μg/mL of GA or vehicle alone (DMSO), and PVR ( a ) and Nectin2 ( b ) surface expression was evaluated on cells gated as in Supplementary Fig. . Data from two representative patients are shown in left panels. Data from 9 patients analysed are shown in right panels. Each dot represents a single patient. ***p < 0.001 Wilcoxon matched pairs test.

Article Snippet: Surface ligands expression on patient-derived PCs was evaluated by means of PE-conjugated anti-PVR mAb (Biolegend, SKII.4), and APC-conjugated anti-Nectin2 mAb (R&D Systems, FAB2229A) after gating on the CD38 + CD138 + PC population.

Techniques: Expressing